HNSCC RNA-seq Dataset Summary
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GEO Accession: GSE65858
Dataset Title: Gene expression patterns and TP53 mutations are associated with HPV RNA status, lymph node metastasis, and survival in head and neck cancer

Dataset Source:
  Original publication: Head and neck cancer cohort
  Total samples in GEO: 270
  Filtered for analysis: 270

Clinical Classification:
  Complete Response: 137 samples
  Progressive Disease: 133 samples
  Class balance: 50.7% CR, 49.3% PD

Expression Data:
  Genes (features): 31330
  Samples: 270
  Data type: RNA-seq expression values
  Normalization: Log2-transformed (verified as already normalized in GEO)

Response Category Definition:
  Complete Response (CR):
    - No progression event during follow-up (pfs_event = FALSE)
    - Represents patients with good treatment response
  Progressive Disease (PD):
    - Progression event occurred (pfs_event = TRUE)
    - Represents patients with disease progression

Note on Indian Cohort:
  This dataset is a general HNSCC cohort (not India-specific).
  No publicly available India-specific HNSCC RNA-seq dataset
  with treatment response data was found in GEO.

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Success Criteria Validation
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✓ Valid HNSCC dataset downloaded: GSE65858
✓ Clinical data filtered for CR/PD: 270 samples
✓ Normalized expression data: 31330 genes × 270 samples
✓ Minimum sample requirement: 270 ≥ 20 ✓
✓ Clinical-expression data matched and aligned